

Software Version	3.16.10



Experiment File Path:	C:\Users\bnext\Documents\Biotek\experiments\20260604-113617-cytation5-pure-timecourse-gfp.xpt
Protocol File Path:	C:\Users\bnext\Documents\Biotek\protocols\cytation5_protocols\cytation5-pure-timecourse-gfp.prt



Plate Number	Plate 1
Date	6/4/2026
Time	11:36:17 AM
Reader Type:	Cytation5
Reader Serial Number:	1705168
Reading Type	Reader

Procedure Details

Plate Type	Greiner 384 SV NoBind AutoMap
Well Selection	Runtime
Eject plate on completion	
Set Temperature	Setpoint 37C, Gradient 0 C
Start Kinetic	Runtime 6:00:00 (HH:MM:SS), Interval 0:05:00, 73 Reads
    Read	GFP-G70
	Fluorescence Endpoint
	Full Plate
	Filter Set 1
	    Excitation: 485/20,  Emission: 528/20
	    Optics: Top,  Gain: 70
	Light Source: Xenon Flash,  Lamp Energy: High,  Standard Dynamic Range
	Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
	Read Height: 10.5 mm
    Read	GFP-Gext
	Fluorescence Endpoint
	Full Plate
	Filter Set 1
	    Excitation: 485/20,  Emission: 528/20
	    Optics: Top,  Gain: extended
	Light Source: Xenon Flash,  Lamp Energy: High,  Extended Dynamic Range
	Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
	Read Height: 10.5 mm
End Kinetic	
Set Temperature	Incubator off

Layout
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24
A																									Well ID
B																									Well ID
C																									Well ID
D																									Well ID
E																									Well ID
F	SPL1	SPL2	SPL3	SPL4	SPL5	SPL6	SPL7	SPL8																	Well ID
G	SPL1	SPL2	SPL3	SPL4	SPL5	SPL6	SPL7	SPL8																	Well ID
H	SPL1	SPL2	SPL3	SPL4	SPL5	SPL6	SPL7	SPL8																	Well ID
I																									Well ID
J																									Well ID
K																									Well ID
L																									Well ID
M																									Well ID
N																									Well ID
O																									Well ID
P																									Well ID

GFP-G70:485,528

Time	T GFP-G70:485,528	F1	F2	F3	F4	F5	F6	F7	F8	G1	G2	G3	G4	G5	G6	G7	G8	H1	H2	H3	H4	H5	H6	H7	H8
0:00:00	37.0	67273	66547	66366	66669	72914	80586	83232	8	66199	67707	67332	68297	75147	79394	87327	7	69734	69013	69615	68485	63077	40215	68620	1
0:05:00	37.0	61295	60913	60786	61873	66441	72681	75784	10	59872	61351	61198	63261	68006	71321	77878	9	63008	62960	64133	63595	55581	35071	61307	9
0:10:00	37.1	57830	57237	57197	59297	61592	67901	72525	14	56533	57818	57807	60226	63122	66361	74413	29	59541	58858	60259	60802	50951	32360	58785	3
0:15:00	37.0	55493	54073	53267	58729	58256	65101	71766	5	54238	54581	53852	59423	59142	63344	72910	11	57014	55580	55570	59653	48241	30809	58128	14
0:20:00	37.0	51707	45917	41785	58153	54221	62406	70876	11	50488	46414	42056	58630	54970	60746	71611	17	52608	46841	42970	58936	44743	29537	57535	15
0:25:00	37.0	44740	32578	24912	57725	49972	59871	70455	7	43678	32912	25049	58241	50469	58414	71368	16	45644	32909	25152	58568	41215	28336	56924	9
0:30:00	37.0	36047	18721	11131	57545	45660	57746	70425	22	35056	18981	11107	57849	46394	56383	71261	0	36524	18584	11004	58512	38093	27236	56885	7
0:35:00	37.0	26529	8855	4308	57372	41567	55583	70181	15	25768	8945	4273	57692	41991	54666	70749	21	26725	8665	4213	57985	34832	26417	56477	15
0:40:00	37.0	17914	3935	2004	57232	37710	53679	70114	9	17447	4001	1970	57554	38071	52415	70563	17	18043	3835	1983	57979	31548	25635	56226	0
0:45:00	37.0	11370	2099	1315	57300	34197	51669	69806	19	11137	2083	1287	57301	34476	50617	70279	23	11455	2050	1337	57867	28677	24819	56153	9
0:50:00	37.1	7137	1452	1099	57278	31077	50222	69897	0	6982	1415	1085	57460	31308	49158	70337	15	7212	1429	1115	57791	26168	24347	56290	17
0:55:00	37.0	4700	1190	1025	56964	28223	48525	69703	2	4624	1199	1000	57173	28467	47439	69951	6	4812	1212	1048	57734	23864	23438	55916	16
1:00:00	37.0	3422	1118	967	56747	26002	47321	69258	24	3394	1124	957	56929	26047	46043	69711	9	3528	1117	983	57634	21962	22860	55855	0
1:05:00	37.0	2833	1059	971	56546	24166	46000	68873	11	2736	1094	944	57116	24363	45052	69604	8	2869	1072	1009	57364	20541	22509	55810	2
1:10:00	37.0	2453	1046	939	56575	22833	45055	69197	8	2425	1059	947	56730	23044	44249	69364	10	2531	1061	975	57583	19489	22099	55676	16
1:15:00	37.0	2332	1061	951	56156	21991	44514	68845	8	2300	1055	926	56601	22104	43510	69190	15	2413	1058	974	56927	18792	21750	55308	2
1:20:00	37.0	2260	1031	948	56262	21345	43984	68682	21	2257	1057	941	56099	21466	43085	69076	11	2328	1036	961	56888	18227	21607	55316	16
1:25:00	37.0	2224	1024	939	56049	21065	43710	68472	8	2187	1018	946	56167	21174	42735	68974	26	2281	1033	967	56563	17982	21449	55009	13
1:30:00	37.0	2199	1039	935	55957	20761	43519	68334	8	2198	1059	959	55947	20873	42504	68495	18	2278	1053	970	56511	17840	21583	54972	19
1:35:00	37.0	2214	1031	950	55971	20695	43372	68140	11	2187	1052	958	55775	20834	42401	68502	5	2300	1031	970	56504	17848	21554	55025	15
1:40:00	37.1	2188	1038	945	55731	20623	43101	68003	1	2193	1036	927	55818	20788	42261	68461	7	2284	1046	973	56314	17676	21465	54756	14
1:45:00	37.0	2199	1027	943	55588	20543	43287	67828	20	2185	1045	930	55670	20685	42510	68564	13	2289	1047	963	56217	17846	21565	54674	7
1:50:00	37.0	2191	1031	932	55482	20469	43099	67719	4	2174	1052	926	55425	20577	42189	68106	0	2282	1033	978	56058	17865	21611	54408	4
1:55:00	37.0	2170	1022	945	55536	20392	43066	67484	26	2171	1039	921	55378	20641	42159	67825	14	2277	1039	970	56051	17793	21562	54591	23
2:00:00	37.0	2202	1037	941	55364	20418	43007	67604	13	2155	1035	966	55495	20477	42005	67791	4	2287	1043	971	55886	17841	21605	54464	8
2:05:00	37.1	2217	1039	931	55240	20324	42932	67128	16	2180	1022	947	55208	20473	41914	67699	13	2290	1047	968	55522	17807	21681	54494	2
2:10:00	37.0	2203	1038	957	55237	20421	43009	67405	15	2200	1039	942	55584	20569	42146	67894	7	2285	1061	989	55920	17973	21715	54651	19
2:15:00	37.0	2217	1043	958	55135	20389	42659	67446	12	2159	1034	958	55355	20504	41931	67691	15	2291	1050	975	55534	17844	21704	54260	16
2:20:00	37.0	2181	1059	948	54952	20382	42766	66880	15	2198	1051	952	55206	20443	41956	67399	11	2272	1052	966	55653	17869	21723	54191	14
2:25:00	37.0	2195	1049	939	54959	20309	42949	67064	9	2179	1047	953	55128	20493	41907	67403	19	2285	1054	976	55489	17709	21763	54096	6
2:30:00	37.0	2201	1033	945	55138	20365	42880	67140	23	2201	1069	966	55409	20404	41799	67510	25	2282	1050	978	55516	17737	21805	53984	14
2:35:00	37.0	2188	1033	962	54763	20282	42418	66906	15	2203	1049	947	54966	20412	41712	67386	4	2273	1066	961	55257	17708	21732	53812	25
2:40:00	37.1	2199	1049	965	54567	20229	42471	66329	12	2187	1054	955	54751	20210	41489	66907	11	2267	1072	997	55298	17652	21860	53756	21
2:45:00	37.0	2202	1042	941	54671	20238	42442	66583	12	2189	1052	959	54780	20309	41351	66722	18	2257	1058	997	55176	17642	21872	53867	14
2:50:00	37.1	2165	1045	964	55044	20202	42415	66487	15	2174	1074	952	54760	20258	41611	66820	13	2269	1068	988	55156	17953	21831	53692	17
2:55:00	37.1	2194	1045	983	54725	20184	42538	66690	12	2228	1053	980	54621	20406	41549	66946	15	2298	1082	994	55265	18027	21910	53453	17
3:00:00	37.0	2219	1070	969	54752	20105	42615	66543	1	2226	1057	964	54611	20238	41440	66839	17	2297	1059	1001	55017	18077	22020	53659	20
0:00:00																									
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0:00:00																									
0:00:00																									

GFP-Gext:485,528

Time	T GFP-Gext:485,528	F1	F2	F3	F4	F5	F6	F7	F8	G1	G2	G3	G4	G5	G6	G7	G8	H1	H2	H3	H4	H5	H6	H7	H8
0:00:13	37.0	610236	601417	602522	599545	657600	730963	748276	103	600013	613970	611622	621028	677018	721700	790143	114	632279	625212	630301	620755	566138	336681	616336	110
0:05:13	37.0	550774	547088	549675	559423	598419	657120	685392	99	542370	554373	556339	574298	616491	645869	707635	98	574669	571046	581471	577586	504387	294349	559542	94
0:10:13	37.0	518867	513376	516174	535085	555395	612363	657333	99	513348	524414	523299	546417	572687	602062	677405	97	538995	536134	547692	551928	463603	270716	532452	101
0:15:13	37.0	502429	486944	477533	529537	524015	588765	651453	117	492056	493519	486826	540972	536940	574141	666918	96	518936	503685	504429	545104	437194	257232	526677	109
0:20:13	37.1	462359	378567	341818	526140	489244	563891	643427	94	454263	416219	344337	532154	499097	550625	659538	119	475634	419765	352128	538659	373083	248335	524426	112
0:25:13	37.1	369555	267426	201872	520993	448020	539258	642189	100	363417	269423	201928	527183	455156	527355	652488	101	409577	267609	202707	531010	342457	237860	515122	101
0:30:13	37.0	296738	151738	88907	519300	378925	520282	638192	91	290426	153737	88341	528377	420625	512194	647463	102	302289	149605	86783	532749	317338	229831	515888	105
0:35:13	37.1	218827	71437	34325	518520	345457	501795	636707	98	211852	71337	33843	523647	350226	491540	642386	101	220406	69506	33732	531829	287581	222934	515742	101
0:40:13	37.0	147324	31890	16534	519501	313486	484670	632915	101	142087	32015	16397	522850	318030	475791	641395	98	148137	31048	16359	528458	262640	214972	512756	97
0:45:13	37.0	92488	17199	10983	514657	284467	464919	629440	105	90729	17158	10907	522297	286629	461789	639791	102	93890	17036	10971	527596	238532	209023	510076	102
0:50:13	37.0	58037	12024	9107	516623	257250	451357	632365	93	56873	12179	9108	520220	260505	445395	638296	100	59162	12088	9386	523540	216353	203856	511908	105
0:55:13	37.1	38631	10048	8435	515163	233513	437362	628711	111	37859	10120	8561	515824	237190	432613	635365	103	39121	10257	8888	522515	198087	196424	508155	101
1:00:13	37.0	28304	9220	8221	509110	215974	423770	628365	92	27842	9479	8289	516649	217637	416881	633619	109	28745	9437	8439	523326	182772	191661	507989	88
1:05:13	37.0	23109	8980	8108	511865	201954	416957	627759	107	23064	9026	8191	514586	202480	376313	634764	95	23898	9217	8390	519136	171438	188948	509216	102
1:10:13	37.0	20846	8831	8153	510113	190919	376874	624557	94	20493	8920	8074	513542	191920	370862	631767	103	21632	8858	8357	519101	160907	185708	504551	104
1:15:13	37.1	19617	8713	7984	510027	183385	373429	621260	101	19484	8812	8018	512085	185569	364611	630251	98	20362	8882	8455	516551	156627	183209	502967	99
1:20:13	37.0	18964	8735	8032	507992	177794	366782	619705	94	18913	8797	8019	510159	180142	362133	627167	97	19913	8904	8097	520016	151777	181200	505015	97
1:25:13	37.0	18703	8707	7964	509370	175520	364892	619004	102	18697	8865	7961	508605	175919	358662	627484	102	19705	8900	8337	513730	150854	180346	503303	115
1:30:13	37.0	18712	8687	8047	503938	174266	363753	619443	108	18665	8854	8023	508771	176154	356532	621504	99	19464	8897	8241	512075	148871	181218	499353	94
1:35:13	37.0	18660	8662	8089	506336	172132	361944	620404	92	18603	8821	8007	510344	174179	356628	624873	94	19355	9024	8390	510523	149251	180784	497733	108
1:40:13	37.0	18633	8638	7935	500378	170527	360458	617995	91	18571	8756	7964	505930	173520	355004	619206	111	19335	8885	8220	509768	147558	180403	494838	108
1:45:13	37.0	18408	8598	8106	502265	171061	361265	616898	101	18438	8778	8009	507136	173808	354029	618143	99	19307	8861	8138	510893	147681	181272	498303	104
1:50:13	37.1	18596	8827	7911	499810	170037	359157	612805	72	18570	8773	8008	506597	172751	352934	618429	94	19586	8975	8264	507771	148558	181881	497106	114
1:55:13	37.1	18532	8733	8022	500403	169958	358807	609308	98	18341	8707	7942	501786	171590	352358	616120	103	19212	8825	8234	510069	148422	181594	497244	90
2:00:13	37.1	18472	8763	8043	502027	170216	358658	608288	107	18423	8891	7954	503447	172319	351856	617071	106	19508	8987	8192	507987	147878	180984	496335	92
2:05:13	37.0	18366	8777	7957	498183	169413	358868	610527	113	18402	8802	8072	503098	172605	352565	613504	98	19233	8894	8293	505621	147877	181566	495312	116
2:10:13	37.0	18531	8720	7977	501187	169640	358988	611665	114	18420	8789	8115	501987	172607	355192	618420	97	19426	8909	8409	508419	148178	183519	498945	82
2:15:13	37.1	18577	8842	7924	497859	169843	356169	612339	81	18524	8869	8042	503524	171684	351955	616956	79	19322	9018	8334	507084	147892	181633	496231	95
2:20:13	37.0	18504	8819	8039	498758	169096	356732	610432	100	18375	8646	8074	502892	171837	351077	614173	105	19262	8884	8298	503392	148069	182375	494711	84
2:25:13	37.0	18505	8757	8008	494558	170173	357233	611434	91	18434	8882	8077	498790	170342	350983	612392	113	19400	8941	8300	504657	148175	183538	492803	90
2:30:13	37.0	18623	8887	8156	497617	169159	357171	609377	96	18525	8830	8088	500328	171978	352256	613385	115	19447	8803	8316	503860	148645	182943	495625	104
2:35:13	37.0	18599	8768	8071	493954	168408	355840	608247	100	18533	8850	8157	499798	170581	350047	612622	94	19320	9085	8501	500279	148208	182543	488837	97
2:40:13	37.0	18489	8829	8055	493183	168421	354973	604917	108	18529	8996	7995	499948	170917	350125	609656	102	19458	9114	8219	502950	147862	182660	490053	111
2:45:13	37.0	18219	8705	8080	495325	168136	356037	602874	92	18457	8902	8053	498848	170296	348293	605512	95	19379	8964	8306	498896	148598	183123	490203	87
2:50:13	37.0	18593	8833	8097	495100	169026	354354	607513	101	18566	9012	8048	497618	170419	349174	611457	105	19291	8988	8466	500781	148394	183631	487792	107
2:55:13	37.0	18706	8795	8172	496651	167923	353715	605434	78	18529	8928	8131	497972	170398	350792	607982	94	19323	8979	8325	498739	148359	184608	489754	103
3:00:13	37.0	18628	8869	8196	493483	168381	355559	607609	85	18627	8956	8231	498772	170531	348998	610635	121	19571	9033	8344	499580	148428	184714	489454	108
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